Title: Optimising Terpene Synthesis with Flow Biocatalysis

Tang X, Allemann RK, Wirth T (2017). Optimising Terpene Synthesis with Flow Biocatalysis. Cardiff University. http://doi.org/10.17035/d.2016.0011675544

Access Rights: Data can be made freely available subject to attribution
Access Method: Click to email a request for this data to opendata@cardiff.ac.uk

Cardiff University Dataset Creators

Dataset Details
Publisher: Cardiff University
Date (year) of data becoming publicly available: 2017
Data format: pdf, jpeg, NMR processing formats
Software Required: NMR processing software (e.g. Topspin, Mnova NMR)
Estimated total storage size of dataset: Less than 1 gigabyte
Number of Files In Dataset: 13300
DOI: 10.17035/d.2016.0011675544


Sesquiterpenes are an important family of natural products that often exhibit important pharmaceutical and agricultural properties. They are biosynthesised from farnesyl diphosphate in sesquiterpene synthase catalysed reactions. Here we report the development of a highly efficient segmented flow system for the enzymatic continuous flow production of sesquiterpenes. Design of experiment (DoE) methods were used to optimise the performance of the flow biocatalysis and quantitative yields were achieved using an operationally simple but highly effective segmented flow system.
All data are provided as pdf: scans were made of the paper-based experimental outputs from various measurement systems. These comprise:
analysis of the reaction mixture using gas chromatography with a Flame Ionization Detector;
analysis of the reaction mixture using gas chromatography with an Electron Impact Mass Spectroscopy detector;
analysis of chemical compound using Nuclear Magnetic Resonance Spectroscopy.
Raw NMR data files are also available and can be opened with suitable software.

Research results based upon these data are published at https://doi.org/10.1002/ejoc.201601388 .


NMR spectra

Related Projects
Flow biocatalysis (20/07/2015 - 14/09/2019)

Last updated on 2019-05-07 at 13:30