Title:    Optimising Terpene Synthesis with Flow Biocatalysis

Citation
Tang X, Allemann RK, Wirth T (2017). Optimising Terpene Synthesis with Flow Biocatalysis. Cardiff University. https://doi.org/10.17035/d.2016.0011675544

Dataset Details

Publisher: Cardiff University

Date (year) of data becoming publicly available: 2017

Data format: pdf, jpeg, NMR processing formats

Software Required: NMR processing software (e.g. Topspin, Mnova NMR)

Estimated total storage size of dataset: Less than 1 gigabyte

Number of Files In Dataset: 13300

DOI : 10.17035/d.2016.0011675544

DOI URL: http://doi.org/10.17035/d.2016.0011675544

Description

Sesquiterpenes are an important family of natural products that often exhibit important pharmaceutical and agricultural properties. They are biosynthesised from farnesyl diphosphate in sesquiterpene synthase catalysed reactions. Here we report the development of a highly efficient segmented flow system for the enzymatic continuous flow production of sesquiterpenes. Design of experiment (DoE) methods were used to optimise the performance of the flow biocatalysis and quantitative yields were achieved using an operationally simple but highly effective segmented flow system.
All data are provided as pdf: scans were made of the paper-based experimental outputs from various measurement systems. These comprise:
analysis of the reaction mixture using gas chromatography with a Flame Ionization Detector;
analysis of the reaction mixture using gas chromatography with an Electron Impact Mass Spectroscopy detector;
analysis of chemical compound using Nuclear Magnetic Resonance Spectroscopy.
Raw NMR data files are also available and can be opened with suitable software.

Research results based upon these data are published at https://doi.org/10.1002/ejoc.201601388 .