Title:    The Occurrence and Zoonotic Potential of Cryptosporidium Species in Freshwater Biota Data

Citation
Robinson Guy, Chalmers Rachel M, Ormerod Steve J, et al.  (2023). The Occurrence and Zoonotic Potential of Cryptosporidium Species in Freshwater Biota Data. Cardiff University. https://doi.org/10.5285/84242834-dc78-49a6-83cb-951edac65d18

Dataset Details

Publisher: Cardiff University

Date (year) of data becoming publicly available: 2023

Data format: .csv

Estimated total storage size of dataset: Less than 100 megabytes

DOI : 10.5285/84242834-dc78-49a6-83cb-951edac65d18

DOI URL: http://doi.org/10.5285/84242834-dc78-49a6-83cb-951edac65d18

Related URL: https://doi.org/10.5285/84242834-dc78-49a6-83cb-951edac65d18

Description

The data comprises of three .csv files (invertebrate larvae and fish/mammalian results as well as site descriptions). The aquatic invertebrate file, entitled Results_Invertebrate_Larvae.csv, is structured with the following headings:

Sample_number describes the unique number given to the sample at the CRU;

Sample_Description describes the type of invertebrate larva tested;

Number_of_larvae_pooled(Seeded_with_C.hominis_oocysts) describes the number of larvae testing in each sample and where larvae were seeded with C. hominis oocysts how many were used;

Site describes the location that the larvae were collected (refer to DURESS_CU_site_description.csv);

n18S describes the result of the nested ssu rRNA PCR(Neg = Negative, Pos = Positive;

Ch_Ct_value describes the Ct value from the C. hominis real-time PCR; IC_Ct value describes the Ct value from the internal control PCR.

The file containing the data from the other sample types, entitled Results_Biofilm_Fish_Mammals.csv, is structured with the following headings:

Site describes the site where the sample was collected (refer to supporting document DURESS_CU_site_description.csv);

Sample_group describes the general source of the sample (e.g. biofilm or fish, etc);

Host describes the source of the sample in more detail (e.g. trout, For biofilm samples N/A stands for not applicable.);

Sample_Type describes the nature of the sample (e.g. biofilm, faeces),

CRU_Ref describes the unique number given to the sample at the CRU; n18S_result describes the result of the nested ssu rRNA PCR and sequenecing;

GP60 describes the result of the nested gp60 PCR

Sequencing describes the result of the sequencing.

Neg = negative, Pos = positive, Not Done = sequencing not carried out

Where the source of the faces could not be identified unambiguously a question mark has been added to the name of the mammal (e.g. Mink?)

Data is available for download from: https://doi.org/10.5285/84242834-dc78-49a6-83cb-951edac65d18