Title: Raw datasets associated with the publication Active Site Loop Engineering Abolishes Water Capture in Hydroxylating Sesquiterpene Synthases
Citation
Srivastava Prabhakar L, Johns Sam T, Walters Rebecca, et al. (2023). Raw datasets associated with the publication Active Site Loop Engineering Abolishes Water Capture in Hydroxylating Sesquiterpene Synthases. Cardiff University. https://doi.org/10.17035/d.2023.0291278347
Access Rights: Creative Commons Attribution 4.0 International
Access Method: https://doi.org/10.17035/d.2023.0291278347 will take you to the repository page for this dataset, where you will be able to download the data or find further access information, as appropriate.
Dataset Details
Publisher: Cardiff University
Date (year) of data becoming publicly available: 2023
Data format: .xlsx, .txt, .ch, .mth, .reg, .MAC, .log, .par, .info, .exe, .temp, .par
Software Required: Gas Chromatography/Mass spectrometry data interpretations software such as MassLynx
NMR spectroscopy processing software such as Mesternova or vNMR
Estimated total storage size of dataset: Less than 100 megabytes
DOI : 10.17035/d.2023.0291278347
DOI URL: http://doi.org/10.17035/d.2023.0291278347
Spreadsheets contating raw data and data analysis for determination of kinetic parameters (Km, kcat) for wlid-type and mutants. (Excel spreadsheets, further details described within the individual spreadsheets). Raw GC-MS outputs for assays to analyse product distribution. Files will need specialist software such as Perkin-Elmer TurboMass and Agilent GC ChemStation to process. Raw NMR spectroscopic data for all compounds generated. (specialist sofware such as Mestrenova or Topsin will be required to process).
Description
Keywords
alpha-bulnesene, enzyme engineering, Hydroxylation, Molecular dynamics, Terpene synthase
Related Projects
- Engineering water capture in terpene synthases (01/03/2018 - 05/03/2022)
