Title: Detection of Polyvinylpyrrolidone in Daphnia magna: Development of a refractive index quantification method for water-soluble polymers in aquatic organisms: data
Citation
Tarring E, Harbottle M, Durance I, et al. (2024). Detection of Polyvinylpyrrolidone in Daphnia magna: Development of a refractive index quantification method for water-soluble polymers in aquatic organisms: data. Cardiff University. https://doi.org/10.17035/d.2024.0318661497
Access Rights: Creative Commons Attribution 4.0 International
Access Method: https://doi.org/10.17035/d.2024.0318661497 will take you to the repository page for this dataset, where you will be able to download the data or find further access information, as appropriate.
Cardiff University Dataset Creators
Dataset Details
Publisher: Cardiff University
Date (year) of data becoming publicly available: 2024
Data format: xlsx, dat, fit
Software Required: Excel, Fityk
Estimated total storage size of dataset: Less than 100 megabytes
DOI : 10.17035/d.2024.0318661497
DOI URL: http://doi.org/10.17035/d.2024.0318661497
The water-soluble polymer polyvinylpyrrolidone (PVP) is an established ingredient in pharmaceutical and personal care product (PPCP) formulations. Due to its high usage and lack of biodegradability, it has been detected up to 7.0 mg L-1 in wastewater and 0.1 mg L-1 in the receiving freshwaters, with several studies showing detrimental sublethal effects in a range of aquatic species. A lack of simple analytical methods to detect and quantify PVP currently impacts further investigation into the cause of these sublethal effects. In this paper we propose a refractive index gel-permeation chromatography (GPC) method to quantify PVP, which includes the processing of raw chromatograms using line deconvolution to calculate peak area. The method was then applied to Daphnia magna exposed to PVP for 48 hours. A limit of detection (LOD) and limit of quantification (LOQ) of 0.05 and 0.2 mg mL-1 respectively was determined, with a recovery of 78% from spiked Daphnia magna. PVP was detected in the samples above the LOD but below the LOQ. This suggests PVP is ingested by Daphnia magna, which warrants further investigation into whether bioaccumulation of PVP could be causing the sublethal effects seen in other studies. The dataset includes GPC data to describe molecular weights of polymers and lineshape deconvolution data in Excel format. Original data files from Fityk are also included. Research results based upon these data are publisehd at https://doi.org/10.1016/j.envpol.2024.123431
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