Teitl: Data underpinning "Reduction of Folate by Dihydrofolate Reductase from Thermotoga maritima"

Hroch L, Hughes RL, Williams T, et al. (2017). Data underpinning "Reduction of Folate by Dihydrofolate Reductase from Thermotoga maritima". Cardiff University. http://doi.org/10.17035/d.2017.0033099548

Hawliau Mynediad: Gall data fod ar gael yn rhad ac am ddim yn amodol ar briodoli

Dull Mynediad: I anfon cais i gael y data hwn, ebostiwch opendata@caerdydd.ac.uk

Manylion y Set Ddata

Cyhoeddwr: Cardiff University

Dyddiad (y flwyddyn) pryd y daeth y data ar gael i'r cyhoedd: 2017

Fformat y data: .inf, .dat, .idx, .sts, .txt

Meddalwedd ofynnol: Waters' MassLynx software is required to view all files.

Amcangyfrif o gyfanswm maint storio'r set ddata: Llai nag 1 terabeit

Nifer y ffeiliau yn y set ddata: 5

DOI : 10.17035/d.2017.0033099548

DOI URL: http://doi.org/10.17035/d.2017.0033099548


We have shown that DHFR from the hyperthermophilic bacterium Thermotoga maritima is able to catalyse reduction of folate to tetrahydrofolate with a similar efficiency to reduction of dihydrofolate under saturating conditions. NMR and mass spectrometry experiments showed no evidence for production of free dihydrofolate during either the EcDHFR- or TmDHFR-catalysed reductions of folate, suggesting that both enzymes perform the two reduction steps without release of the partially reduced substrate.

Herein we include underpinning liquid chromatography - mass spectrometry data for this publication, acquired using EPSRC-funded equipment.

Researc results based upon these data are published at http://doi.org/10.1021/acs.biochem.6b01268

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Diweddarwyd y tro diwethaf ar 2019-22-07 am 15:47