Title: Data underpinning "Reduction of Folate by Dihydrofolate Reductase from Thermotoga maritima"

Citation
Hroch L, Hughes RL, Williams T, et al. (2017). Data underpinning "Reduction of Folate by Dihydrofolate Reductase from Thermotoga maritima". Cardiff University. http://doi.org/10.17035/d.2017.0033099548


Access Rights: Data can be made freely available subject to attribution
Access Method: Click to email a request for this data to opendata@cardiff.ac.uk

Dataset Details
Publisher: Cardiff University
Date (year) of data becoming publicly available: 2017
Data format: .inf, .dat, .idx, .sts, .txt
Software Required: Waters' MassLynx software is required to view all files.
Estimated total storage size of dataset: Less than 1 terabyte
Number of Files In Dataset: 5
DOI: 10.17035/d.2017.0033099548

Description

We have shown that DHFR from the hyperthermophilic bacterium Thermotoga maritima is able to catalyse reduction of folate to tetrahydrofolate with a similar efficiency to reduction of dihydrofolate under saturating conditions. NMR and mass spectrometry experiments showed no evidence for production of free dihydrofolate during either the EcDHFR- or TmDHFR-catalysed reductions of folate, suggesting that both enzymes perform the two reduction steps without release of the partially reduced substrate.

Herein we include underpinning liquid chromatography - mass spectrometry data for this publication, acquired using EPSRC-funded equipment.

Researc results based upon these data are published at  http://doi.org/10.1021/acs.biochem.6b01268



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Last updated on 2019-22-07 at 15:47