Title: Data underpinning "Reduction of Folate by Dihydrofolate Reductase from Thermotoga maritima"
CitationHroch L, Hughes RL, Williams T, et al. (2017). Data underpinning "Reduction of Folate by Dihydrofolate Reductase from Thermotoga maritima". Cardiff University. http://doi.org/10.17035/d.2017.0033099548
Access Rights: Data can be made freely available subject to attribution
Cardiff University Dataset Creators
Dataset Details
Publisher: Cardiff University
Date (year) of data becoming publicly available: 2017
Data format: .inf, .dat, .idx, .sts, .txt
Software Required: Waters' MassLynx software is required to view all files.
Estimated total storage size of dataset: Less than 1 terabyte
Number of Files In Dataset: 5
DOI: 10.17035/d.2017.0033099548
DescriptionWe have shown that DHFR from the hyperthermophilic bacterium Thermotoga maritima is able to catalyse reduction of folate to tetrahydrofolate with a similar efficiency to reduction of dihydrofolate under saturating conditions. NMR and mass spectrometry experiments showed no evidence for production of free dihydrofolate during either the EcDHFR- or TmDHFR-catalysed reductions of folate, suggesting that both enzymes perform the two reduction steps without release of the partially reduced substrate.
Herein we include underpinning liquid chromatography - mass spectrometry data for this publication, acquired using EPSRC-funded equipment.
Researc results based upon these data are published at http://doi.org/10.1021/acs.biochem.6b01268