Title: Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle

Citation
Zaki AJ, Thomas SK, Watson P, et al. (2018). Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle. Cardiff University. https://doi.org/10.17035/d.2018.0045008721

Dataset Details

Publisher: Cardiff University

Date (year) of data becoming publicly available: 2018

Coverage start date: 01/03/2014

Coverage end date: 27/11/2017

Data format: .czi, .000, .txt

Software Required: Gwyddion (open source) or other SPM software for Nanoscope III SPM data.
Python-bioformats for CZI Image format for microscopes or other.

Estimated total storage size of dataset: Less than 100 megabytes

DOI : 10.17035/d.2018.0045008721

DOI URL: http://doi.org/10.17035/d.2018.0045008721

Description

We have created modified protein variants by introducing a non-canonical amino acid p-azido-L-phenylalanine (azF) into a defined positions for photochemically-induced covalent attachment to graphene. Attachment of GFP, TEM and cyt b 562 proteins was verified through a combination of atomic force and scanning tunnelling microscopy, resistance measurements, Raman data and fluorescence measurements. This method can in principle be extended to any protein which can be engineered in this way without adversely affecting its structural stability.

Data files used in this publication include

AFM and STM files

Raman data (used for figure 11 and in the supporting information)

Confocal microscopy data files (used for figure 5 fluorescence images)

Research results based upon these data are published at http://doi.org/10.1039/C7RA11166E