Teitl: Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle

Dyfyniad
Zaki AJ, Thomas SK, Watson P, et al. (2018). Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle. Cardiff University. http://doi.org/10.17035/d.2018.0045008721


Hawliau Mynediad: Gall data fod ar gael yn rhad ac am ddim yn amodol ar briodoli
Dull Mynediad: I anfon cais i gael y data hwn, ebostiwch opendata@caerdydd.ac.uk

Manylion y Set Ddata
Cyhoeddwr: Cardiff University
Dyddiad (y flwyddyn) pryd y daeth y data ar gael i'r cyhoedd: 2018
Dyddiad dechrau creu'r data: 01.03.2014
Dyddiad gorffen creu'r data: 27.11.2017
Fformat y data: .czi, .000, .txt
Meddalwedd ofynnol: Gwyddion (open source) or other SPM software for Nanoscope III SPM data.
Python-bioformats for CZI Image format for microscopes or other.

Amcangyfrif o gyfanswm maint storio'r set ddata: Llai na 100 megabeit
DOI: 10.17035/d.2018.0045008721

Disgrifiad

We have created modified protein variants by introducing a non-canonical amino acid p-azido-L-phenylalanine (azF) into a defined positions for photochemically-induced covalent attachment to graphene. Attachment of GFP, TEM and cyt b 562 proteins was verified through a combination of atomic force and scanning tunnelling microscopy, resistance measurements, Raman data and fluorescence measurements. This method can in principle be extended to any protein which can be engineered in this way without adversely affecting its structural stability.

Data files used in this publication include

AFM and STM files

Raman data (used for figure 11 and in the supporting information)

Confocal microscopy data files (used for figure 5 fluorescence images)

Research results based upon these data are published at http://doi.org/10.1039/C7RA11166E



Prosiectau Cysylltiedig

Diweddarwyd y tro diwethaf ar 2019-25-11 am 12:30