Title: Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle

Citation
Zaki AJ, Thomas SK, Watson P, et al. (2018). Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle. Cardiff University. http://doi.org/10.17035/d.2018.0045008721


Access Rights: Data can be made freely available subject to attribution
Access Method: Click to email a request for this data to opendata@cardiff.ac.uk

Dataset Details
Publisher: Cardiff University
Date (year) of data becoming publicly available: 2018
Coverage start date: 01/03/2014
Coverage end date: 27/11/2017
Data format: .czi, .000, .txt
Software Required: Gwyddion (open source) or other SPM software for Nanoscope III SPM data.
Python-bioformats for CZI Image format for microscopes or other.

Estimated total storage size of dataset: Less than 100 megabytes
DOI: 10.17035/d.2018.0045008721

Description

We have created modified protein variants by introducing a non-canonical amino acid p-azido-L-phenylalanine (azF) into a defined positions for photochemically-induced covalent attachment to graphene. Attachment of GFP, TEM and cyt b 562 proteins was verified through a combination of atomic force and scanning tunnelling microscopy, resistance measurements, Raman data and fluorescence measurements. This method can in principle be extended to any protein which can be engineered in this way without adversely affecting its structural stability.

Data files used in this publication include

AFM and STM files

Raman data (used for figure 11 and in the supporting information)

Confocal microscopy data files (used for figure 5 fluorescence images)

Research results based upon these data are published at http://doi.org/10.1039/C7RA11166E



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Last updated on 2019-25-11 at 12:30