Title: Different cell-penetrating peptides (CPPs) for delivery of green fluorescent protein (GFP) into mammalian cells

Citation
Patel SG, Sayers EJ, He L, et al. (2018). Different cell-penetrating peptides (CPPs) for delivery of green fluorescent protein (GFP) into mammalian cells. Cardiff University. https://doi.org/10.17035/d.2018.0064167981

Dataset Details

Publisher: Cardiff University

Date (year) of data becoming publicly available: 2018

Coverage start date: 01/11/2016

Coverage end date: 31/10/2018

Data format: .lif, .fcs

Software Required: ImageJ and ProSort were used to view .lif and .fcs files, respectively

Estimated total storage size of dataset: Less than 1 terabyte

DOI : 10.17035/d.2018.0064167981

DOI URL: http://doi.org/10.17035/d.2018.0064167981

Description

We use green fluorescent protein (GFP) as a model cargo to compare the delivery capability of five CPP sequences (Penetratin, R8, TAT, Transportan, Xentry) and their derivatives (cyclic R8, cyclic TAT, TAT-HA) in four cell lines (HeLa, HEK, 10T1/2, HepG2).

For confocal microscopy experiments, HeLa cells were incubated with 10 µM of the designated eGFP-CPP fusion proteins at 37 °C for 1 h before imaging. Nucleus were stained with Hoechst which shows blue fluorescence. Biological duplicates of confocal microscopy results (*.lif) are available here.

For flow cytometry experiments, cells were treated with 10 µM of eGFP-CPP fusion proteins at 37 °C for 1 h before analysis. Biological triplicates of flow cytometry results (*.fcs) are available here.