Teitl: Different cell-penetrating peptides (CPPs) for delivery of green fluorescent protein (GFP) into mammalian cells
Dyfyniad
Patel SG, Sayers EJ, He L, et al. (2018). Different cell-penetrating peptides (CPPs) for delivery of green fluorescent protein (GFP) into mammalian cells. Cardiff University. https://doi.org/10.17035/d.2018.0064167981
Nid yw'r data hwn ar gael ar hyn o bryd oherwydd: Bwriad i gyhoeddi canlyniadau prosiect
Hawliau Mynediad: Gall data fod ar gael yn rhad ac am ddim yn amodol ar briodoli
Dull Mynediad: I anfon cais i gael y data hwn, ebostiwch opendata@caerdydd.ac.uk
Crewyr y Set Ddata o Brifysgol Caerdydd
Manylion y Set Ddata
Cyhoeddwr: Cardiff University
Dyddiad (y flwyddyn) pryd y daeth y data ar gael i'r cyhoedd: 2018
Dyddiad dechrau creu'r data: 01.11.2016
Dyddiad gorffen creu'r data: 31.10.2018
Fformat y data: .lif, .fcs
Meddalwedd ofynnol: ImageJ and ProSort were used to view .lif and .fcs files, respectively
Amcangyfrif o gyfanswm maint storio'r set ddata: Llai nag 1 terabeit
DOI : 10.17035/d.2018.0064167981
DOI URL: http://doi.org/10.17035/d.2018.0064167981
We use green fluorescent protein (GFP) as a model cargo to compare the delivery capability of five CPP sequences (Penetratin, R8, TAT, Transportan, Xentry) and their derivatives (cyclic R8, cyclic TAT, TAT-HA) in four cell lines (HeLa, HEK, 10T1/2, HepG2). For confocal microscopy experiments, HeLa cells were incubated with 10 µM of the designated eGFP-CPP fusion proteins at 37 °C for 1 h before imaging. Nucleus were stained with Hoechst which shows blue fluorescence. Biological duplicates of confocal microscopy results (*.lif) are available here. For flow cytometry experiments, cells were treated with 10 µM of eGFP-CPP fusion proteins at 37 °C for 1 h before analysis. Biological triplicates of flow cytometry results (*.fcs) are available here.
Disgrifiad
Prosiectau Cysylltiedig
- Cleavable linkers for traceless delivery of therapeutics into cells (01.09.2016 - 31.03.2017)
- Traceless non-invasive and spatiotemporal control of protein activity in cells (01.07.2017 - 31.12.2020)