Title: Data set for paper: Positive functional synergy of structurally integrated artificial protein dimers assembled by Click chemistry


Citation
Worthy HL, Auhim HS, Jamieson WD, et al. (2019). Data set for paper: Positive functional synergy of structurally integrated artificial protein dimers assembled by Click chemistry. Cardiff University. http://doi.org/10.17035/d.2019.0078708057



Access Rights: Data is provided under a Creative Commons Attribution (CC BY 4.0) licence

Access Method: Click to email a request for this data to opendata@cardiff.ac.uk


Dataset Details

Publisher: Cardiff University

Date (year) of data becoming publicly available: 2019

Data format: png, xlsx

Software Required: Microsoft Excel

Estimated total storage size of dataset: Less than 100 megabytes

Number of Files In Dataset: 7

DOI : 10.17035/d.2019.0078708057

DOI URL: http://doi.org/10.17035/d.2019.0078708057


Description

The dataset for the spectral data (absorbance and fluoresence) presented in the paper together with the full gel images that consistute Supporting Figure 4. All structural information has been submitted to the PDB with accession code 5nhn.

Steady state fluoresence was performed as described in the experimental section of the related manuscript. The datasets for absorbance has given in molar absorbance coefficient so as to standardise to a protein concentrtation of 1 M. The methods for determining protein concentration are highlighted in the associated paper. Importantly, we used sfGFP as the reference for generating standard curves of concentration. We also used a molar absorbance coeffficent of 49000 M-1cm-1 at 485 nm as the originally reported value is wrong. The fluoresence data is presented largely as normalised data. Included in the fluoresence dataset is the data of fluoresence intnesity (in arbitory units) against wavelength used to normalise related spectra; the data point used as the normalisation point of 1 is highlighted also.

Research results based upon these data are published at https://doi.org/10.1038/s42004-019-0185-5


Keywords

Fluorescence Spectroscopy, Protein engineering, Structure elucidation

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Last updated on 2021-05-07 at 11:45