Teitl: Label-free quantitative chemical imaging and classification analysis of adipogenesis in mouse embryonic stem cells

Dyfyniad
Masia F, Langbein WW, Borri P (2017). Label-free quantitative chemical imaging and classification analysis of adipogenesis in mouse embryonic stem cells. Cardiff University. https://doi.org/10.17035/d.2017.0009096459

Manylion y Set Ddata

Cyhoeddwr: Cardiff University

Dyddiad (y flwyddyn) pryd y daeth y data ar gael i'r cyhoedd: 2017

Fformat y data: .tif, .dat

Meddalwedd ofynnol: ImageJ

Amcangyfrif o gyfanswm maint storio'r set ddata: Llai nag 1 terabeit

Nifer y ffeiliau yn y set ddata: 10

DOI : 10.17035/d.2017.0009096459

DOI URL: http://doi.org/10.17035/d.2017.0009096459

Disgrifiad

The data contains coherent anti-Stokes Raman scattering hyperspectral images of differentiated murine embryonic stem cells and their analysis in terms of concentrations of chemical components and their spectra using the hyperspectral image analysis (HIA) software developed by ourselves. Chemical decomposition into protein, lipid and aqueous components combined with a statistical analysis of their spatial patterns enabled us to extract a set parameters that were used for classification analysis. Additionally the correlation between the analysed CARS images and fluorescence images showing the distribution of FAB protein 4 is also reported. Finally the co-localization between the lipid and protein components is discussed.

Research results based upon these data are published at https://doi.org/10.1002/jbio.201700219

Meysydd Ymchwil

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• Confocal microscopy
• Embryonic stem cells
• High-throughput image analysis
• Lasers - Nonlinear Optics
• Microscopy - Optical